Difference between revisions of "Part:BBa K395303"
 
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<partinfo>BBa_K395303 short</partinfo>
 
<partinfo>BBa_K395303 short</partinfo>
  
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Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. This promoter is a truncated version of BBa_R0082.  
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This part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of the upstream of P''OmpC(WT)''BBa_R0082 which originally comprises 3 distinct phosphorylated OmpR recognition sites, binding site 1, binding site 2 and binding site 3. ''OmpC(CS1)'' promoter consists of only binding site 1 before the canonical -35 and -10 region. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about P''ompC'' series]
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[[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|Figure 1. Induction of new'' OmpC'' series in high osmolarity medium at 4 hours. We characterized this promoter by attaching GFP(E0240) to the downstream and mearsuring its transcriptional activity through the GFP expression. This work is done by Taichi NAKAMURA Tokyo Tech and Thiprampai THAMAMONGOOD iGEM2010.]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 00:23, 28 October 2010

PompC(CS1) Promoter regulated by phosphorylated OmpR

This part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system. The EnvZ-OmpR system of E. coli regulates the transcriptional activity of this promoter in response to change in extracellular osmolarity. This promoter is a derivatives of the upstream of POmpC(WT)BBa_R0082 which originally comprises 3 distinct phosphorylated OmpR recognition sites, binding site 1, binding site 2 and binding site 3. OmpC(CS1) promoter consists of only binding site 1 before the canonical -35 and -10 region. [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about PompC series]

Figure 1. Induction of new OmpC series in high osmolarity medium at 4 hours. We characterized this promoter by attaching GFP(E0240) to the downstream and mearsuring its transcriptional activity through the GFP expression. This work is done by Taichi NAKAMURA Tokyo Tech and Thiprampai THAMAMONGOOD iGEM2010.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]