Difference between revisions of "Part:BBa K395306"

Latest revision as of 00:22, 28 October 2010

PompC(CS1) with GFP reporter (K395303+E0240)

This part is contructed for measuring the transcriptional activity of OmpC(CS1) promoter through the GFP expression. The promoter of this part is positively regulated by phosphorylated OmpR, a response regulator in EnvZ-OmpR two-component system [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about PompC series]

Figure 1. Induction of new OmpC series in high osmolarity medium at 4 hours. This work is done by Taichi Nakamura and Thiprampai THAMAMONGOOD
Tokyo Tech iGEM2010

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 730

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K395306 short</partinfo>
-Positively regulated, OmpR-controlled promoter. This promoter is derived from the upstream region of ompC. Phosphorylated OmpR binds to the operator site and activates transcription. In this biobrick, this promoter is a truncated version of BBa_R0082.
-This biobrick report with GFP, whether sequence was read or not.
+This part is contructed for measuring the transcriptional activity of [https://parts.igem.org/Part:BBa_K395303 ''OmpC(CS1)''] promoter through the GFP expression. The promoter of this part is positively regulated by phosphorylated ''OmpR'', a response regulator in  EnvZ-''OmpR'' two-component system [http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about P''ompC'' series]
+[[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|Figure 1. Induction of new'' OmpC'' series in high osmolarity medium at 4 hours. This work is done by Taichi Nakamura and Thiprampai THAMAMONGOOD  <br/>Tokyo Tech iGEM2010 ]]
 <!-- Add more about the biology of this part here