Difference between revisions of "Part:BBa K416002:Design"

 
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<partinfo>BBa_K416002 short</partinfo>
 
<partinfo>BBa_K416002 short</partinfo>
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===Design Notes===
 
===Design Notes===
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The coding region of the gene was isolated via PCR. Biobrick prefix and suffix were added during amplification, and ligated into Ampicillin resistant plasmids.
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This is a LoxP site which is the target of Cre Recombinase. It is normally 34-bp, but we added 2bp to the 3' end so that the part can be assembled within a transcribed region of our system. The 2bp added do not cause a stop codon and should not interfere with transcription.
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===Source===
 
===Source===
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We used the 34-bp LoxP Site from the 2010 iGEM initial distribution, then rebiobricked it using a reverse primer that contained the extra 2bp.
  
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Amplified and biobricked from E. coli.
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===References===
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Sauer, Brian. Functional Expression of the ''cre-lox'' Site-Specific Recombination System in the Yeast ''Saccharmoyces cerevisiae''.
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Molecular and Cellular Biology, June 1987, p. 2087-2096
  
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===References===
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Nagy, Andras. Cre recombinase: The universal reagent for genome tailoring.
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Genesis, 26: p. 99-109

Latest revision as of 00:12, 28 October 2010

36 Base Pair LoxP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is a LoxP site which is the target of Cre Recombinase. It is normally 34-bp, but we added 2bp to the 3' end so that the part can be assembled within a transcribed region of our system. The 2bp added do not cause a stop codon and should not interfere with transcription.

Source

We used the 34-bp LoxP Site from the 2010 iGEM initial distribution, then rebiobricked it using a reverse primer that contained the extra 2bp.

References

Sauer, Brian. Functional Expression of the cre-lox Site-Specific Recombination System in the Yeast Saccharmoyces cerevisiae. Molecular and Cellular Biology, June 1987, p. 2087-2096

Nagy, Andras. Cre recombinase: The universal reagent for genome tailoring. Genesis, 26: p. 99-109