Difference between revisions of "Part:BBa K395705"

Latest revision as of 00:11, 28 October 2010

crtW (beta-carotene ketolase) with rbs

CrtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.

This is the enzyme of the carotenoid biosynthesis pathway. CrtW converts zeaxanthin to astaxanthin, the final metabolite of the pathway.

Fig. 2-1 carotenoid biosynthesis pathway, astaxanthin plate astaxanthin extract, and TLC
This work is done by Yumiko Kinoshita

In order to confirm that BBa_K395705 is worked, we construct a BioBrick part (BBa_K395706), which contains this part under BBa_K395702. We synthesized astaxanthin by BBa_K395706, when add beta-carotene as a substrate. Thus we confirmed that BBa_395705 is working. Characterization of BBa_K395706 has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 632
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 715
Illegal SapI site found at 542
Illegal SapI site found at 554

@@ Line 4: / Line 4: @@
 CrtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.
-Part of the carotenoid biosynthesis pathway. CrtW converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin.
+This is the enzyme of the carotenoid biosynthesis pathway. CrtW converts zeaxanthin to astaxanthin, the final metabolite of the pathway.
-[[Image:tokyotech_Fig. 2-1-11-2 astaxanthin pellet and plate.jpg|right|460px|Fig. 2-1-11-2 astaxanthin pellet and plate]]
+[[Image:Tokyotech registry-asta.jpg|left|700px|thumb|Fig. 2-1 carotenoid biosynthesis pathway, astaxanthin plate astaxanthin extract, and TLC<br>This work is done by Yumiko Kinoshita]]
-[[Image:tokyotech_Fig. 2-1-12-2 astaxanthin extract.jpg|right|500px|Fig. 2-1-12-2 astaxanthin extract]]
-[[Image:tokyotech_table. 2.1.3.jpg|left|450px|figure2]]
-[[Image:tokyotech_Fig. 2-1-1-3.TLC.jpg|left|350px|Fig. 2-1-1-3.TLC.jpg]]
@@ Line 26: / Line 23: @@
-These parts contain ''crtW'' (&beta;-carotene ketolase) under inducible promoter control. This enzyme is responsible for the conversion of zeaxanthin into astaxanthin. Our team designed two BioBricks related to this conversion. Characterization of BBa_K395706 has been performed in ''E. coli'' strain MG1655.   (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])
@@ Line 52: / Line 48: @@
+In order to confirm that BBa_K395705 is worked, we construct a BioBrick part (BBa_K395706), which contains this part under BBa_K395702. We synthesized astaxanthin by BBa_K395706, when add beta-carotene as a substrate. Thus we confirmed that BBa_395705 is working. Characterization of BBa_K395706 has been performed in E. coli strain MG1655.   (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])
@@ Line 62: / Line 59: @@
 <!-- Add more about the biology of this part here
 ===Usage and Biology===