Difference between revisions of "Part:BBa K395704"

Revision as of 23:44, 27 October 2010

zeaxanthin synthesis operon (pBad+rbs+crtZ+plac+rbs +crtEBIY )

This part contains all five genes required, CrtEBIYZ, to produce zeaxanthin.

It is able to control the expression of crtZ by arabinose.this part applies to ①

Fig.2 zeaxanthin pellet and plate, TLC
These works are done by Yumiko Kinoshita

These parts contain crtZ (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
Illegal BamHI site found at 3810
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 3346
Illegal NgoMIV site found at 3476
Illegal AgeI site found at 979
Illegal AgeI site found at 2561
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

@@ Line 6: / Line 6: @@
 It is able to control the expression of crtZ by arabinose.this part applies to ①
-[[Image:tokyotech_Fig. 1.1.8.1 zeaxanthin pellet and plate.jpg|right|460px|Fig. 1.1.8.1 zeaxanthin pellet and plate]]
+[[Image:Tokyotech_registry_zeaxanthin.jpg|center|500px|thumb|Fig.2 zeaxanthin pellet and plate, TLC<br>These works are done by Yumiko Kinoshita]]
-[[Image:tokyotech_table. 1.1.1.2.jpg|left|450px|figure1]]
-[[Image:tokyotech_Fig. 2-1-1-3.TLC.jpg|left|450px|Fig. 2-1-1-3.TLC.jpg]]
 These parts contain ''crtZ'' (&beta;-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of &beta;-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in ''E. coli'' strain MG1655.  (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])