Difference between revisions of "Part:BBa K404124:Design"
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===Source=== | ===Source=== | ||
| − | + | mGMK_TK30: Herpes simplex virus<br /> | |
| − | + | phTERT : Human telomerase promoter <br /> | |
| + | Misc: pAAV_MCS (Stratagene) | ||
===References=== | ===References=== | ||
Latest revision as of 23:14, 27 October 2010
[AAV2]-left-ITR_phTERT_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1122
Illegal NgoMIV site found at 1780
Illegal NgoMIV site found at 2685
Illegal AgeI site found at 2814 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3216
Illegal SapI.rc site found at 1172
Design Notes
All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard. Fusion of two parts was conducted following the proposed BioBrick Assembly Strategy (https://parts.igem.org/Assembly:Standard_assembly). Since the gene of interest was designed in the RFC25 standard, BioBrick assembly leads to a scar and the additional restriction enzymes NgoMIV and AgeI.
Source
mGMK_TK30: Herpes simplex virus
phTERT : Human telomerase promoter
Misc: pAAV_MCS (Stratagene)
References
Reference
Cech, T., 2000. Life at the End of the Chromosome: Telomeres and Telomerase. Angewandte Chemie (International ed. in English), 39(1), 34-43. Available at: http://www.ncbi.nlm.nih.gov/pubmed/10649348.
Kyo, S. et al., 2008. Understanding and exploiting hTERT promoter regulation for diagnosis and treatment of human cancers. Cancer science, 99(8), 1528-38. Available at: http://www.ncbi.nlm.nih.gov/pubmed/18754863.
Wick, M., Zubov, D. & Hagen, G., 1999. Genomic organization and promoter characterization of the gene encoding the human telomerase reverse transcriptase (hTERT). Gene, 232(1), 97-106. Available at: http://www.ncbi.nlm.nih.gov/pubmed/10333526.