Difference between revisions of "Part:BBa K322120:Design"

 
 
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<partinfo>BBa_K322120 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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CcaS and PhoR combine to be the sensor biobrick and PhoR and LacZ combine to be the biobrick of reporter part.  
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CcaS and PhoR fusion forms the green light sensor. PhoR and LacZ act as a reporter system. There are still two illegal restriction sites in the sequence.
  
  

Latest revision as of 23:10, 27 October 2010

Green light sensor with LacZ reporter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2640
    Illegal SpeI site found at 2735
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2640
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2011
    Illegal SpeI site found at 2735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2640
    Illegal BamHI site found at 644
    Illegal XhoI site found at 2948
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2640
    Illegal SpeI site found at 2735
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2640
    Illegal SpeI site found at 2735
    Illegal NgoMIV site found at 971
    Illegal AgeI site found at 2675
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CcaS and PhoR fusion forms the green light sensor. PhoR and LacZ act as a reporter system. There are still two illegal restriction sites in the sequence.


Source

CcaS: Synechocystis sp. (strain PCC 6803)- Synthesised by Unam Mexico 2010 PhoR: Escherichia coli (strain K12)genomic DNA PhoA promoter: Escherichia coli K12 LacZ reporter: Escherichia coli K12


References