Difference between revisions of "Part:BBa K389001"

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<partinfo>BBa_K389001 short</partinfo>
 
<partinfo>BBa_K389001 short</partinfo>
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[[Image:Vira_rezeptor.jpg|300px|thumb|right|The VirA receptor of ''Agrobacterium tumefaciens'']]
 
The VirA receptor is used by ''Agrobacterium tumefaciens'' to detect acetosyringone and other phenolic substances which are secreted by plants after injury. In presence of these substances VirA phosphorylates itself and afterwards VirG, a response regulator which activates ''vir'' promoters. These promoters control genes which are used for infecting the injured plant.  
 
The VirA receptor is used by ''Agrobacterium tumefaciens'' to detect acetosyringone and other phenolic substances which are secreted by plants after injury. In presence of these substances VirA phosphorylates itself and afterwards VirG, a response regulator which activates ''vir'' promoters. These promoters control genes which are used for infecting the injured plant.  
  
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===Usage and Biology===
 
===Usage and Biology===
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For a working VirA/G-receptor system, you also need the ''virG'' BioBrick <partinfo>K389002</partinfo> and a ''vir'' promoter <partinfo>K389003</partinfo>. You can find an already assembled VirA/G-receptor system here: <partinfo>K389017</partinfo>.  
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For a working VirA/G-receptor system, you also need the ''virG'' BioBrick <partinfo>K389002</partinfo> and a ''vir'' promoter <partinfo>K389003</partinfo>.  
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You can find an already assembled VirA/G-receptor system here: <partinfo>K389017</partinfo>.  
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====Specifity====
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The functionality and specifity of this BioBrick were tested with the devices: <partinfo>K389015</partinfo>, <partinfo>K389016</partinfo>.
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This receptor recognizes:
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* Acetosyringone
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This receptor does not recognize
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* Capsaicin
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* Dopamine
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* Homovanillic acid
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* 3-Methoxytyramine
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This receptor recognizes acetosyringone.
 
 
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Latest revision as of 22:34, 27 October 2010

VirA receptor

The VirA receptor of Agrobacterium tumefaciens

The VirA receptor is used by Agrobacterium tumefaciens to detect acetosyringone and other phenolic substances which are secreted by plants after injury. In presence of these substances VirA phosphorylates itself and afterwards VirG, a response regulator which activates vir promoters. These promoters control genes which are used for infecting the injured plant.

This virA gene was isolated from the TI-plasmid of A. tumefaciens C58. An illegal PstI restriction site was removed by site-directed mutagenesis.


Usage and Biology

For a working VirA/G-receptor system, you also need the virG BioBrick BBa_K389002 and a vir promoter BBa_K389003.

You can find an already assembled VirA/G-receptor system here: BBa_K389017.


Specifity

The functionality and specifity of this BioBrick were tested with the devices: BBa_K389015, BBa_K389016.

This receptor recognizes:

  • Acetosyringone

This receptor does not recognize

  • Capsaicin
  • Dopamine
  • Homovanillic acid
  • 3-Methoxytyramine


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 586
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1571
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1707