Difference between revisions of "Part:BBa K361011"
Line 11: Line 11:
 
!Promotor
 
!Promotor
 
!lac promotor
 
!lac promotor
 +
|-
 +
!RBS efficiency
 +
!1
 +
|-
 +
!Rci site specific recombinase
 +
![[Part:BBa_K361000|'''BBa_K361000''']]
 +
|-
 +
!terminator
 +
!double bidirectional terminator
 
|}
 
|}
 
</center>
 
</center>
−
 
−
 
−
*promotor: lac promotor
 
−
*RBS: define efficiency =1
 
−
*Rci site specific recombinase: please refer </partinfo> BBa_K361000 </partinfo>
 
−
*terminator: double bidirectional terminator
 
  
 
Here is our system leading to generation of Rci site-specific recombinase (yellow in colour)
 
Here is our system leading to generation of Rci site-specific recombinase (yellow in colour)

Revision as of 22:13, 27 October 2010

Rci recombinase system

This is the protein genertors of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor and a ribosome binding site in front of it, a double terminator after it.


Performance

Promotor lac promotor
RBS efficiency 1
Rci site specific recombinase BBa_K361000
terminator double bidirectional terminator

Here is our system leading to generation of Rci site-specific recombinase (yellow in colour) Rci sys.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 588
    Illegal NgoMIV site found at 1083
  • 1000
    COMPATIBLE WITH RFC[1000]