Difference between revisions of "Part:BBa K361011"
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This is the protein genertors of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor and a ribosome binding site in front of it, a double terminator after it.
 
This is the protein genertors of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor and a ribosome binding site in front of it, a double terminator after it.
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'''Performance'''<br>
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<center>
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{|{{Table}}
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!Promotor
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!lac promotor
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|}
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</center>
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*promotor: lac promotor
 
*promotor: lac promotor

Revision as of 22:08, 27 October 2010

Rci recombinase system

This is the protein genertors of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor and a ribosome binding site in front of it, a double terminator after it.


Performance

Promotor lac promotor


  • promotor: lac promotor
  • RBS: define efficiency =1
  • Rci site specific recombinase: please refer </partinfo> BBa_K361000 </partinfo>
  • terminator: double bidirectional terminator

Here is our system leading to generation of Rci site-specific recombinase (yellow in colour) Rci sys.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 588
    Illegal NgoMIV site found at 1083
  • 1000
    COMPATIBLE WITH RFC[1000]