Difference between revisions of "Part:BBa K389412"

Revision as of 21:43, 27 October 2010

vir promoter + sensitivity tuner + luciferase

This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.

By modifying the Sensitivity Tuner the RBS from the phage activator from the original Sensitivity Tuner was removed. New RBS comes with Virb-promotor element K389003.

Usage and Biology

For a working VirA/G-receptor system, you also need the virA BioBrick BBa_K389001. You can find the complete working system here: BBa_K389422

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2428
Illegal NgoMIV site found at 3772
Illegal NgoMIV site found at 3793
Illegal AgeI site found at 1951
Illegal AgeI site found at 2063
Illegal AgeI site found at 2265
Illegal AgeI site found at 3496
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 3678

@@ Line 6: / Line 6: @@
 By modifying the Sensitivity Tuner the RBS from the phage activator from the original Sensitivity Tuner was  removed. New RBS comes with Virb-promotor element [https://parts.igem.org/cgi/sequencing/one_blast.cgi?id=7888 K389003].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+For a working VirA/G-receptor system, you also need the ''virA'' BioBrick <partinfo>K389001</partinfo>. You can find the complete working system here: <partinfo>K389422</partinfo>
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