Difference between revisions of "Part:BBa K361011"
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*Rci site specific recombinase: please refer </partinfo> BBa_K361000 </partinfo> | *Rci site specific recombinase: please refer </partinfo> BBa_K361000 </partinfo> | ||
*terminator: double bidirectional terminator | *terminator: double bidirectional terminator | ||
| + | |||
| + | Here is our system leading to production of Rci site-specific recombinase (yellow in colour) | ||
| + | [[Image:rci_sys.jpg]] | ||
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Revision as of 21:33, 27 October 2010
Rci recombinase system
This is the protein genertors of Rci site-specific recombinase BBa_K361000, which is being linked with a promotor and a ribosome binding site in front of it, a double terminator after it.
- promotor: lac promotor
- RBS: define efficiency =1
- Rci site specific recombinase: please refer </partinfo> BBa_K361000 </partinfo>
- terminator: double bidirectional terminator
Here is our system leading to production of Rci site-specific recombinase (yellow in colour) File:Rci sys.jpg
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 588
Illegal NgoMIV site found at 1083 - 1000COMPATIBLE WITH RFC[1000]