Difference between revisions of "Part:BBa K319041:Design"
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===Backgournd=== | ===Backgournd=== | ||
− | The nat gene was first isolated from Streptomyces noursei | + | The nat gene was first isolated from Streptomyces noursei[1]. This gene provides the organism with resistance to the antibiotic nourseothricin. In 1999, Goldstein and McCusker produced a nat cassette based on the kanMX cassettes of plasmid pFA6 and showed that this new cassette confers resistance to the above mentioned drug in ''S. cerevisiae'' strain YAG44[2]. We have BioBricked the complete cassette (including the upstream TEF1 promoter and the downstream TEF1 terminator) and have shown that it has 100% selectivity in ''S. cerevisiae'' YPH500 strain. |
===Source=== | ===Source=== |
Revision as of 21:11, 27 October 2010
NatMX cassette
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 690
Illegal NgoMIV site found at 779
Illegal AgeI site found at 408 - 1000COMPATIBLE WITH RFC[1000]
Backgournd
The nat gene was first isolated from Streptomyces noursei[1]. This gene provides the organism with resistance to the antibiotic nourseothricin. In 1999, Goldstein and McCusker produced a nat cassette based on the kanMX cassettes of plasmid pFA6 and showed that this new cassette confers resistance to the above mentioned drug in S. cerevisiae strain YAG44[2]. We have BioBricked the complete cassette (including the upstream TEF1 promoter and the downstream TEF1 terminator) and have shown that it has 100% selectivity in S. cerevisiae YPH500 strain.
Source
n