Difference between revisions of "Part:BBa K317003:Design"
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<partinfo>BBa_K317003 short</partinfo> | <partinfo>BBa_K317003 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication | + | The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication |
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| + | [[Image:Phototaxis Device-2.png|400px]] | ||
| + | [[Image:Primer sets.png|400px]] | ||
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | Jung,et al.(2001)J.Bacteriol.183,6365–6371 | ||
Latest revision as of 21:11, 27 October 2010
NpSRII-9a.a.linker_NpHtrII-StTar
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1789
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 73
Illegal NgoMIV site found at 331
Illegal AgeI site found at 1591 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1027
Illegal BsaI.rc site found at 1306
Illegal SapI site found at 801
Illegal SapI.rc site found at 1807
Design Notes
The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication
Source
Natronomonas pharaonis (Obtained from National Institute of Technology and Evaluation(NBRC). (NBRC 14720T)) Salmonella Typhimurium
References
Jung,et al.(2001)J.Bacteriol.183,6365–6371