Difference between revisions of "Part:BBa K404012"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K404012 short</partinfo>
 
<partinfo>BBa_K404012 short</partinfo>
  
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{| style="color:black; margin: 0px 0px 500px 20px;" cellpadding="6" cellspacing="1" border="2" align="right"
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! colspan="2" style="background:#66bbff;"|[https://parts.igem.org/Part:BBa_K404012 AAV2-VP3]
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|-
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|'''BioBrick Nr.'''
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|[https://parts.igem.org/Part:BBa_K404012 BBa_K404012]
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|-
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|'''RFC standard'''
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|[https://parts.igem.org/Help:Assembly_standard_25 RFC 25]
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|-
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|'''Requirement'''
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|pSB1C3_001<br>
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|-
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|'''Source'''
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|pAAV_RC from Stratagene
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|-
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|'''Submitted by'''
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|[http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]
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|}
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<html>
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The viral capsid is formed by the three structural proteins VP1, VP2 and VP3 which are encoded by the cap gene in an overlapping reading frame. They form an icosahedral symmetry arranged in a stoichiometric ratio of 1:1:10. The mRNA encoding for the cap proteins is transcribed from p40 and alternative spliced to minor and major products. Sharing a common C terminus and stop codon, the VP proteins begin with a different start codon.
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<h3>References</h3>
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<b>DiPrimio, Asokan, Govindasamy, Agbandje-McKenna, & Samulski</b>, June 2008. Surface loop dynamics in adeno-associated virus capsid assembly. Journal of virology, 167(1), 5178–5189 <br />
  
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<center><img src="https://static.igem.org/mediawiki/parts/a/a7/Freiburg10_Cap_proteins_VP1_2%263.png" width="600"
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height="auto" margin: 10px 10px 10px 10px/></center>
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<b> Figure 1: The VP proteins are encoded in an overlapping open reading frame. </b>.
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<br/>
  
  
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</html>
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===Usage and Biology===
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Latest revision as of 21:05, 27 October 2010

[AAV2]-VP3

AAV2-VP3
BioBrick Nr. BBa_K404012
RFC standard RFC 25
Requirement pSB1C3_001
Source pAAV_RC from Stratagene
Submitted by [http://2010.igem.org/Team:Freiburg_Bioware FreiGEM 2010]

The viral capsid is formed by the three structural proteins VP1, VP2 and VP3 which are encoded by the cap gene in an overlapping reading frame. They form an icosahedral symmetry arranged in a stoichiometric ratio of 1:1:10. The mRNA encoding for the cap proteins is transcribed from p40 and alternative spliced to minor and major products. Sharing a common C terminus and stop codon, the VP proteins begin with a different start codon.

References

DiPrimio, Asokan, Govindasamy, Agbandje-McKenna, & Samulski, June 2008. Surface loop dynamics in adeno-associated virus capsid assembly. Journal of virology, 167(1), 5178–5189
Figure 1: The VP proteins are encoded in an overlapping open reading frame. .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 560