Difference between revisions of "Part:BBa K346013"

Latest revision as of 20:46, 27 October 2010

Constutitive promoter(BBa_J23114)+RBS+MerR(BBa_K346001)

This part is composed of the constutitive promoter BBa_J23109 and a transcription factor merR. MerR can activate PmerT as a dimer in the presence of Hg(II) in a dose-response manner. We used the structure along with PmerT-RBS-GFP(BBa_K346096) to see how GFP expression vary in certain concentration of MerR when indcued by different concentraton of the Hg(II). For more information about merR, please click RBS-merR(BBa_K346001)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K346013 short</partinfo>
-This part is composed of constutitive promoter BBa_J23109 and transcription factor MerR. It can regulate PmerT to represent a dose-response manner to response to Hg(II) concentration.
+This part is composed of the constutitive promoter BBa_J23109 and a transcription factor merR. MerR can activate PmerT as a dimer in the presence of Hg(II) in a dose-response manner. We used the structure along with PmerT-RBS-GFP(BBa_K346096) to see how GFP expression vary in certain concentration of MerR when indcued by different concentraton of the Hg(II). For more information about merR, please click [https://parts.igem.org/Part:BBa_K346001 RBS-merR(BBa_K346001)]
-We used the structure along with PmerT-RBS-GFP to see under the constant MerR concentration level how GFP expression vary according to the Hg(II) concentration.
 [[Image:6.jpg]]