Difference between revisions of "Part:BBa K404122:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K404122 short</partinfo>
 
<partinfo>BBa_K404122 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
x
+
All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard. Fusion of two parts was conducted following the proposed BioBrick Assembly Strategy (https://parts.igem.org/Assembly:Standard_assembly). Since the gene of interest was designed according to RFC25, the BioBrick assembly created cloning scars up- and downstream of the GOI, mVenus.
  
 +
===Source===
 +
Since this composite part consists of several BioBrick parts, several sources exist.
 +
As for the suicide gene, mGMK_TK was isolated from the herpes simplex virus (HSV). Addtionally, the elements for efficient viral particles production were obtained from pAAV_MCS.
  
 +
===References===
 +
<html>
 +
<p style='text-indent:36.0pt'><span lang=EN-US style='font-size:10.0pt;
 +
font-family:"Calibri","sans-serif"'>Ardiani, A., Sanchez-Bonilla, M. &amp;
 +
Black, M.E., 2010. Fusion enzymes containing HSV-1 thymidine kinase mutants and
 +
guanylate kinase enhance prodrug sensitivity in vitro and in vivo. <i>Cancer
 +
gene therapy</i>, 17(2), 86-96. Available at:
 +
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2808426&amp;tool=pmcentrez&amp;rendertype=abstract.</span></p>
  
−
===Source===
+
<p style='text-indent:36.0pt'><span lang=EN-US style='font-size:10.0pt;
 +
font-family:"Calibri","sans-serif"'>Black, M.E. et al., 1996. Creation of
 +
drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene
 +
therapy. <i>Proceedings of the National Academy of Sciences of the United
 +
States of America</i>, 93(8), 3525-9. Available at:
 +
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=39643&amp;tool=pmcentrez&amp;rendertype=abstract.</span></p>
  
−
x
+
<p style='text-indent:36.0pt'><span lang=EN-US style='font-size:10.0pt;
 +
font-family:"Calibri","sans-serif"'>Fuchita, M. et al., 2009. Bacterial
 +
cytosine deaminase mutants created by molecular engineering show improved
 +
5-fluorocytosine-mediated cell killing in vitro and in vivo. <i>Cancer research</i>,
 +
69(11), 4791-9. Available at:
 +
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2765227&amp;tool=pmcentrez&amp;rendertype=abstract.</span></p>
  
−
===References===
+
<p style='text-indent:36.0pt'><span lang=EN-US style='font-size:10.0pt;
 +
font-family:"Calibri","sans-serif"'>Fuchita, M. et al., 2009. Bacterial
 +
cytosine deaminase mutants created by molecular engineering show improved
 +
5-fluorocytosine-mediated cell killing in vitro and in vivo. <i>Cancer research</i>,
 +
69(11), 4791-9. Available at: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2765227&amp;tool=pmcentrez&amp;rendertype=abstract.</span></p>
 +
 
 +
<p style='text-indent:36.0pt'><span style='font-size:10.0pt;font-family:"Calibri","sans-serif"'>Greco,
 +
O. &amp; Dachs, G.U., 2001. </span><span lang=EN-US style='font-size:10.0pt;
 +
font-family:"Calibri","sans-serif"'>Gene directed enzyme/prodrug therapy of
 +
cancer: historical appraisal and future prospectives. <i>Journal of cellular
 +
physiology</i>, 187(1), 22-36. Available at:
 +
http://www.ncbi.nlm.nih.gov/pubmed/11241346.</span></p>
 +
 
 +
<p style='text-indent:36.0pt'><span style='font-size:10.0pt;font-family:"Calibri","sans-serif"'>Huber,
 +
B.E. et al., 1993. </span><span lang=EN-US style='font-size:10.0pt;font-family:
 +
"Calibri","sans-serif"'>In vivo antitumor activity of 5-fluorocytosine on human
 +
colorectal carcinoma cells genetically modified to express cytosine deaminase. <i>Cancer
 +
research</i>, 53(19), 4619-26. Available at:
 +
http://www.ncbi.nlm.nih.gov/pubmed/8402637.</span></p>
 +
 
 +
<p style='text-indent:36.0pt'><span style='font-size:10.0pt;font-family:"Calibri","sans-serif"'>Huber,
 +
B.E. et al., 1994. </span><span lang=EN-US style='font-size:10.0pt;font-family:
 +
"Calibri","sans-serif"'>Metabolism of 5-fluorocytosine to 5-fluorouracil in
 +
human colorectal tumor cells transduced with the cytosine deaminase gene:
 +
significant antitumor effects when only a small percentage of tumor cells
 +
express cytosine deaminase. <i>Proceedings of the National Academy of Sciences
 +
of the United States of America</i>, 91(17), 8302-6. Available at:
 +
http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=44594&amp;tool=pmcentrez&amp;rendertype=abstract.</span></p>
 +
 
 +
<p style='text-indent:36.0pt'><span lang=EN-US style='font-size:10.0pt;
 +
font-family:"Calibri","sans-serif"'>Moolten, F.L., 1986. Tumor chemosensitivity
 +
conferred by inserted herpes thymidine kinase genes: paradigm for a prospective
 +
cancer control strategy. <i>Cancer research</i>, 46(10), 5276-81. Available at:
 +
http://www.ncbi.nlm.nih.gov/pubmed/3019523.</span></p>
 +
 
 +
<p style='text-indent:36.0pt'><span lang=EN-US style='font-size:10.0pt;
 +
font-family:"Calibri","sans-serif"'>Roche, <i>Apoptosis , Cell Death and Cell
 +
Proliferation</i>,</span></p>
 +
 
 +
<p style='text-indent:36.0pt'><span lang=EN-US style='font-size:10.0pt;
 +
font-family:"Calibri","sans-serif"'>Willmon, C.L., Krabbenhoft, E. &amp; Black,
 +
M.E., 2006. A guanylate kinase/HSV-1 thymidine kinase fusion protein enhances
 +
prodrug-mediated cell killing. <i>Gene therapy</i>, 13(17), 1309-12. Available
 +
at: http://www.ncbi.nlm.nih.gov/pubmed/16810197.</span></p>
 +
 
 +
</html>

Latest revision as of 20:42, 27 October 2010

[AAV2]-left-ITR_pCMV_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1319
    Illegal NgoMIV site found at 1977
    Illegal NgoMIV site found at 2882
    Illegal AgeI site found at 3011
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3413
    Illegal SapI.rc site found at 1369


Design Notes

All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard. Fusion of two parts was conducted following the proposed BioBrick Assembly Strategy (https://parts.igem.org/Assembly:Standard_assembly). Since the gene of interest was designed according to RFC25, the BioBrick assembly created cloning scars up- and downstream of the GOI, mVenus.

Source

Since this composite part consists of several BioBrick parts, several sources exist. As for the suicide gene, mGMK_TK was isolated from the herpes simplex virus (HSV). Addtionally, the elements for efficient viral particles production were obtained from pAAV_MCS.

References

Ardiani, A., Sanchez-Bonilla, M. & Black, M.E., 2010. Fusion enzymes containing HSV-1 thymidine kinase mutants and guanylate kinase enhance prodrug sensitivity in vitro and in vivo. Cancer gene therapy, 17(2), 86-96. Available at: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2808426&tool=pmcentrez&rendertype=abstract.

Black, M.E. et al., 1996. Creation of drug-specific herpes simplex virus type 1 thymidine kinase mutants for gene therapy. Proceedings of the National Academy of Sciences of the United States of America, 93(8), 3525-9. Available at: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=39643&tool=pmcentrez&rendertype=abstract.

Fuchita, M. et al., 2009. Bacterial cytosine deaminase mutants created by molecular engineering show improved 5-fluorocytosine-mediated cell killing in vitro and in vivo. Cancer research, 69(11), 4791-9. Available at: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2765227&tool=pmcentrez&rendertype=abstract.

Fuchita, M. et al., 2009. Bacterial cytosine deaminase mutants created by molecular engineering show improved 5-fluorocytosine-mediated cell killing in vitro and in vivo. Cancer research, 69(11), 4791-9. Available at: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=2765227&tool=pmcentrez&rendertype=abstract.

Greco, O. & Dachs, G.U., 2001. Gene directed enzyme/prodrug therapy of cancer: historical appraisal and future prospectives. Journal of cellular physiology, 187(1), 22-36. Available at: http://www.ncbi.nlm.nih.gov/pubmed/11241346.

Huber, B.E. et al., 1993. In vivo antitumor activity of 5-fluorocytosine on human colorectal carcinoma cells genetically modified to express cytosine deaminase. Cancer research, 53(19), 4619-26. Available at: http://www.ncbi.nlm.nih.gov/pubmed/8402637.

Huber, B.E. et al., 1994. Metabolism of 5-fluorocytosine to 5-fluorouracil in human colorectal tumor cells transduced with the cytosine deaminase gene: significant antitumor effects when only a small percentage of tumor cells express cytosine deaminase. Proceedings of the National Academy of Sciences of the United States of America, 91(17), 8302-6. Available at: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=44594&tool=pmcentrez&rendertype=abstract.

Moolten, F.L., 1986. Tumor chemosensitivity conferred by inserted herpes thymidine kinase genes: paradigm for a prospective cancer control strategy. Cancer research, 46(10), 5276-81. Available at: http://www.ncbi.nlm.nih.gov/pubmed/3019523.

Roche, Apoptosis , Cell Death and Cell Proliferation,

Willmon, C.L., Krabbenhoft, E. & Black, M.E., 2006. A guanylate kinase/HSV-1 thymidine kinase fusion protein enhances prodrug-mediated cell killing. Gene therapy, 13(17), 1309-12. Available at: http://www.ncbi.nlm.nih.gov/pubmed/16810197.