Difference between revisions of "Part:BBa K249004"

 
 
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<partinfo>BBa_K249004 short</partinfo>
 
<partinfo>BBa_K249004 short</partinfo>
  
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pSB1A3 with an N-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly. Adds 10 Arginine residues to the N-terminus of a Protein.
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pSB1A3 with an N-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly. Adds 10 Arginine residues to the N-terminus of a Protein (includes Start Codon AUG).
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K249004 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K249004 SequenceAndFeatures</partinfo>
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This part was designed as a plasmid vector backbone and was expected to work by adding an N-terminal Arginine Tag to the protein to which it would be fused.
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N-terminal tagged YFP did not have substantially more fluorescence than control cells. Cells expressing C-terminal tagged YFP had ten times more fluorescence than control cells and cells expressing N-terminal tagged YFP.
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<img src="https://static.igem.org/mediawiki/parts/3/3e/UofLNvsC-YFP1.jpg" width="450"/>
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<img src="https://static.igem.org/mediawiki/parts/6/62/UofLNvsC-YFP2.jpg" width="450"/>
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Latest revision as of 20:35, 27 October 2010

N-terminal Arginine fusion vector

pSB1A3 with an N-terminal Arginine tag. This particular plasmid utilizes the BioFusion (aka Silver Lab) Standard for BioBrick assembly. Adds 10 Arginine residues to the N-terminus of a Protein (includes Start Codon AUG).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


This part was designed as a plasmid vector backbone and was expected to work by adding an N-terminal Arginine Tag to the protein to which it would be fused.

N-terminal tagged YFP did not have substantially more fluorescence than control cells. Cells expressing C-terminal tagged YFP had ten times more fluorescence than control cells and cells expressing N-terminal tagged YFP.