Difference between revisions of "Part:BBa K323005:Design"

(→‎Design Notes)
(→‎Source)
 
(3 intermediate revisions by 2 users not shown)
Line 8: Line 8:
  
 
===Source===
 
===Source===
−
 
+
Sequence of zinc finger PBSII was synthesised by GeneArt, whereas sequence of YFP is commercially available.
−
a
+
  
 
===References===
 
===References===
 +
 +
Kerppola T.K., Hu C. 2003. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nature biotechnology (21): 539-545
 +
 +
Nurmemmedov E, Thunnissen M. 2006. Expression, purification, and characterization of the 4 zinc finger region of human tumor suppressor WT1. Protein Expr Purif.;46 (2): 379-89

Latest revision as of 20:34, 27 October 2010

CMV ATG PBSII link nYFP stop - part of mammalian split/FRET system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Sequence of zinc finger PBSII was synthesised by GeneArt, whereas sequence of YFP is commercially available.

References

Kerppola T.K., Hu C. 2003. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nature biotechnology (21): 539-545

Nurmemmedov E, Thunnissen M. 2006. Expression, purification, and characterization of the 4 zinc finger region of human tumor suppressor WT1. Protein Expr Purif.;46 (2): 379-89