Difference between revisions of "Part:BBa K323029:Design"

(→‎Design Notes)
(→‎Source)
 
(2 intermediate revisions by 2 users not shown)
Line 8: Line 8:
  
 
===Source===
 
===Source===
−
 
+
Cyan fluorescent protein (CFP) was obtained from a commercially available vectror C1. HivC sequence was already deposited in a Registry as [https://parts.igem.org/Part:BBa_K165006 BBa_K165006].
−
a
+
  
 
===References===
 
===References===
 +
 +
Kerppola T.K., Hu C. 2003. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nature biotechnology (21): 539-545
 +
 +
Nurmemmedov E, Thunnissen M. 2006. Expression, purification, and characterization of the 4 zinc finger region of human tumor suppressor WT1. Protein Expr Purif.;46 (2): 379-89

Latest revision as of 20:25, 27 October 2010

CMV ATG cCFP link ZNF HIVC His stop - part of mammalian split/FRET system


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1026
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Cyan fluorescent protein (CFP) was obtained from a commercially available vectror C1. HivC sequence was already deposited in a Registry as BBa_K165006.

References

Kerppola T.K., Hu C. 2003. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nature biotechnology (21): 539-545

Nurmemmedov E, Thunnissen M. 2006. Expression, purification, and characterization of the 4 zinc finger region of human tumor suppressor WT1. Protein Expr Purif.;46 (2): 379-89