Difference between revisions of "Part:BBa K325219:ArabinosetoLight"
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'''Description'''<br> | '''Description'''<br> | ||
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| + | This page describes the relationship between Arabinose concentration in the medium with light output. We used a [http://www.bmglabtech.com/products/microplate-reader/instruments.cfm?product_id=2 FLUOstar OPTIMA] microplate reader to quantify the light output. Protocol and plate reader settings used are given below. | ||
| − | ''' | + | ==Data== |
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| + | [[Image:IntegratedactiPP+LRE.png|thumb|569px|center|'''Figure 1 - Transfer function of <partinfo>K325219</partinfo>. The data points represent the mean of 11 values obtained for light output at 30 min interval from 450 min to 750 min after injection of D-Luciferin. These values are the mean of 3 readings as shown in Figure 3. The corresponding error bars represent an interval of twice the standard deviation across the 33 data points centred around the mean value. ''']] | ||
| + | [[Image:MaximumlumPP+LRE.png|thumb|569px|center|'''Figure 2 - Maximum luminescence output of <partinfo>K325219</partinfo> as a function of Arabinose concentration. These values are the mean of 3 readings as shown in Figure 3. The corresponding error bars represent an interval of twice the standard deviation across the 3 data points centred around the mean value. ''']] | ||
| + | [[Image:TimePP+LRE.png|thumb|569px|center|'''Figure 3 - Evolution of luminescence with time at different Arabinose concentrations. The interval between measurements is 30 min. Mean values and error bars are based on 3 time repeats.''']] | ||
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| − | + | |[[Media:BBa_K325219ArabinosetoLight.xls]] | |
| − | + | |Raw data from experiment | |
| − | + | |21/10/2010 | |
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| + | </div>Measured by the [http://2010.igem.org/Team:Cambridge Cambridge iGEM team 2010] | ||
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'''Compatibility'''<br> | '''Compatibility'''<br> | ||
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'''References'''<br> | '''References'''<br> | ||
[http://www.ncbi.nlm.nih.gov/pubmed/18949818 '''[1]:'''] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,''Life'' '''61''', 6-17. | [http://www.ncbi.nlm.nih.gov/pubmed/18949818 '''[1]:'''] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,''Life'' '''61''', 6-17. | ||
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[http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html '''[2]:'''] T. Nakatsu ''et al.'' (2006) Structural Basis for the spectral difference in luciferase bioluminescence, ''Nature'' '''440'''(16), 372-376. | [http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html '''[2]:'''] T. Nakatsu ''et al.'' (2006) Structural Basis for the spectral difference in luciferase bioluminescence, ''Nature'' '''440'''(16), 372-376. | ||
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[http://www.ncbi.nlm.nih.gov/pubmed/11457857 '''[3]:'''] K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, ''The Journal of Biological Chemistry'', '''276'''(39), 36508-36513. | [http://www.ncbi.nlm.nih.gov/pubmed/11457857 '''[3]:'''] K. Gomi and N. Kajiyama, (2001) Oxyluciferin, a Luminescence Product of Firefly Luciferase, Is Enzymatically Regenerated into Luciferin, ''The Journal of Biological Chemistry'', '''276'''(39), 36508-36513. | ||
Latest revision as of 20:22, 27 October 2010
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Description
This page describes the relationship between Arabinose concentration in the medium with light output. We used a [http://www.bmglabtech.com/products/microplate-reader/instruments.cfm?product_id=2 FLUOstar OPTIMA] microplate reader to quantify the light output. Protocol and plate reader settings used are given below.
Data
Figure 1 - Transfer function of BBa_K325219. The data points represent the mean of 11 values obtained for light output at 30 min interval from 450 min to 750 min after injection of D-Luciferin. These values are the mean of 3 readings as shown in Figure 3. The corresponding error bars represent an interval of twice the standard deviation across the 33 data points centred around the mean value.
Figure 2 - Maximum luminescence output of BBa_K325219 as a function of Arabinose concentration. These values are the mean of 3 readings as shown in Figure 3. The corresponding error bars represent an interval of twice the standard deviation across the 3 data points centred around the mean value.
| Data | Notes | Date Uploaded |
|---|---|---|
| Media:BBa_K325219ArabinosetoLight.xls | Raw data from experiment | 21/10/2010 |
Compatibility
Chassis: Device has been shown to work in Top 10 (Invitrogen)
Plasmids: Device has been shown to work on pSB1C3
References
[http://www.ncbi.nlm.nih.gov/pubmed/18949818 [1]:] S.M. Marques and J.C.G. Esteves da Silva, (2009) Firefly Bioluminescence: A Mechanistic Approach of Luciferase Catalyzed Reactions,Life 61, 6-17.
[http://www.nature.com/nature/journal/v440/n7082/abs/nature04542.html [2]:] T. Nakatsu et al. (2006) Structural Basis for the spectral difference in luciferase bioluminescence, Nature 440(16), 372-376.