Difference between revisions of "Part:BBa K352017:Experience"
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<br> We have tried another construct pCooM-RBS-RFP-TT-pLac-RBS-CooA at TOP10 strain. The results were successful. We have put our construct in different environments (flasks with different conditions) and compared the RFP fluorescence.  
 
<br> We have tried another construct pCooM-RBS-RFP-TT-pLac-RBS-CooA at TOP10 strain. The results were successful. We have put our construct in different environments (flasks with different conditions) and compared the RFP fluorescence.  
  
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<br> The data shows that RFP fluorescence increases with CO induction.  
 
<br> The data shows that RFP fluorescence increases with CO induction.  
  
 
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== Imaging with confocal laser scanning microscopy ==
 
== Imaging with confocal laser scanning microscopy ==
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<div align="center" style="margin:15px 0px 0px 0px"><a><img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/a/ae/Confocal_sol.JPG"></a></div>  
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<div align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/a/ae/Confocal_sol.JPG"></a></div>  
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<div align="center" style="margin:15px 0px 0px 0px"> <img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/2/26/Confocal_sa%C4%9F.JPG"></a></div>  
  
  

Revision as of 18:37, 27 October 2010



Signal Quantification of pCooM-RBS-RFP-TT-pLac-RBS-CooA:


We have tried another construct pCooM-RBS-RFP-TT-pLac-RBS-CooA at TOP10 strain. The results were successful. We have put our construct in different environments (flasks with different conditions) and compared the RFP fluorescence.

w7

The data shows that RFP fluorescence increases with CO induction.


Imaging with confocal laser scanning microscopy

<img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="Confocal_sol.JPG"></a>
<img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="Confocal_sa%C4%9F.JPG"></a>


Applications of BBa_K352017

User Reviews

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