Difference between revisions of "Part:BBa K352017:Experience"
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<br> We have tried another construct pCooM-RBS-RFP-TT-pLac-RBS-CooA at TOP10 strain. The results were successful. We have put our construct in different environments (flasks with different conditions) and compared the RFP fluorescence. | <br> We have tried another construct pCooM-RBS-RFP-TT-pLac-RBS-CooA at TOP10 strain. The results were successful. We have put our construct in different environments (flasks with different conditions) and compared the RFP fluorescence. | ||
| − | <div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/b/b6/BU_SEFER_TAMAM.JPG"></a></div> | + | <div align="center" style="margin:15px 0px 0px 0px"><a><img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/b/b6/BU_SEFER_TAMAM.JPG"></a></div> |
<br> The data shows that RFP fluorescence increases with CO induction. | <br> The data shows that RFP fluorescence increases with CO induction. | ||
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== Imaging with confocal laser scanning microscopy == | == Imaging with confocal laser scanning microscopy == | ||
| − | <div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/a/ae/Confocal_sol.JPG"></a></div> | + | <div align="center" style="margin:15px 0px 0px 0px"><a><img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/a/ae/Confocal_sol.JPG"></a></div> |
| − | <div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/2/26/Confocal_sa%C4%9F.JPG"></a></div> | + | <div align="center" style="margin:15px 0px 0px 0px"><a><img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/2/26/Confocal_sa%C4%9F.JPG"></a></div> |
Revision as of 18:36, 27 October 2010
Signal Quantification of pCooM-RBS-RFP-TT-pLac-RBS-CooA:
We have tried another construct pCooM-RBS-RFP-TT-pLac-RBS-CooA at TOP10 strain. The results were successful. We have put our construct in different environments (flasks with different conditions) and compared the RFP fluorescence.
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The data shows that RFP fluorescence increases with CO induction.
Imaging with confocal laser scanning microscopy
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Applications of BBa_K352017
User Reviews
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