Difference between revisions of "Part:BBa K352017:Experience"
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<br> The data shows that RFP fluorescence increases with CO induction.  
 
<br> The data shows that RFP fluorescence increases with CO induction.  
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== Imaging with confocal laser scanning microscopy ==
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<div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/a/ae/Confocal_sol.JPG"></a></div>
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<div align="center" style="margin:15px 0px 0px 0px"><img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="https://static.igem.org/mediawiki/2010/2/26/Confocal_sa%C4%9F.JPG"></a></div>
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Revision as of 18:35, 27 October 2010



Signal Quantification of pCooM-RBS-RFP-TT-pLac-RBS-CooA:


We have tried another construct pCooM-RBS-RFP-TT-pLac-RBS-CooA at TOP10 strain. The results were successful. We have put our construct in different environments (flasks with different conditions) and compared the RFP fluorescence.

<img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="BU_SEFER_TAMAM.JPG"></a>


The data shows that RFP fluorescence increases with CO induction.


Imaging with confocal laser scanning microscopy

<img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="Confocal_sol.JPG"></a>
<img style="border: 0px solid ; width: 300px; height: 300px;" alt="w7" src="Confocal_sa%C4%9F.JPG"></a>


Applications of BBa_K352017

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