Difference between revisions of "Part:BBa K322921:Experience"

(Applications of BBa_K322921)
(Applications of BBa_K322921)
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===Applications of BBa_K322921===
 
===Applications of BBa_K322921===
characterization:
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Characterisation of the activity of the ''sacB'' BioBrick:
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[[Image:SDC10484.jpg | 600px]]
 
[[Image:SDC10484.jpg | 600px]]
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The control strain which does not contain ''sacB'' has grown well on both plates, whereas the ''sacB''-containing strain X2 grows poorly on 10% sucrose and E6 does not grow at all. This experiment confirms that ''sacB'' is effective as a negative selection marker within the BRIDGE protocol.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 18:23, 27 October 2010

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Please enter how you used this part and how it worked out.

Applications of BBa_K322921

Characterisation of the activity of the sacB BioBrick:


SDC10484.jpg


The control strain which does not contain sacB has grown well on both plates, whereas the sacB-containing strain X2 grows poorly on 10% sucrose and E6 does not grow at all. This experiment confirms that sacB is effective as a negative selection marker within the BRIDGE protocol.

User Reviews

UNIQ27386eff92c2a7ea-partinfo-00000000-QINU UNIQ27386eff92c2a7ea-partinfo-00000001-QINU