Difference between revisions of "Part:BBa K320005"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K320005 short</partinfo> | <partinfo>BBa_K320005 short</partinfo> | ||
− | + | This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(<partinfo>BBa_K320002</partinfo>) and a CFP biobrick (<partinfo>BBa_E0020</partinfo>). | |
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+ | This BioBrick was built to test the constitutive promoter (<partinfo>BBa_K320002</partinfo>). | ||
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+ | It has only been characterized in ''E. coli'' (see the [https://parts.igem.org/Part:BBa_K320005:Experience Experience]). We are currently running experiments to characterize it in ''Asaia'' to continue building gear for our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia new chassis]. | ||
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Latest revision as of 18:21, 27 October 2010
Constitutive expression of CFP
This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(BBa_K320002) and a CFP biobrick (BBa_E0020).
This BioBrick was built to test the constitutive promoter (BBa_K320002).
It has only been characterized in E. coli (see the Experience). We are currently running experiments to characterize it in Asaia to continue building gear for our [http://2010.igem.org/Team:EPF_Lausanne/Project_asaia new chassis].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 62
Illegal NheI site found at 146 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]