Difference between revisions of "Part:BBa K431008"
Line 3: Line 3:
  
 
pTEF
 
pTEF
−
The TEF1- α gene is comprised of 1.380 kB and encodes a polypeptide of 459 amino acids and a molecular weight of 50.1 kDa. The final product is the soluble protein Translational Elongation Factor. This protein has been determined to be one of the most abundant proteins in eukaryotic cells and known to be associated with cell growth. Recognizing the high level of the protein present in the cell, it was predicted that TEF1- α is under the control of a strong promoter. It was discovered that the translational elongation factor is under the control of strong constitutive promoter pTEF.
+
The TEF1- α gene is comprised of 1.380 kB and encodes a polypeptide of 459 amino acids and a molecular weight of 50.1 kDa. The final product is the soluble protein Translational Elongation Factor. This protein has been determined to be one of the most abundant proteins in eukaryotic cells and known to be associated with cell growth. Recognizing the high level of the protein present in the cell, it was predicted that TEF1- α is under the control of a strong promoter. It was discovered that the translational elongation factor is under the control of strong constitutive promoter pTEF.pTEF was then isolated and compared with another strong constitutive promoter, glyceraldehydes-3-phosphate dehydrogenase promoter (pGAP).  According to research done on this promoter it has been determine that when compared to pGAP, pTEF was shown to work well in high glucose environments and carbon-limited conditions, therefore leading to similar or even greater expression levels of recombinant protein.  It was also determined that pTEF does not require an inducer, since it is a constitutive promoter.  pTEF can also be used in the mass production of heterologuous proteins.  From the research done on this promoter it was concluded that pTEF can provide more choices on production conditions and easier methods for expression than pGAP.  Therefore, pTEF would be a great alternative for expressing foreign genes in Pichia pastoris for industrial application.  This promoter sequence can be isolated from yeast Pichia pastoris.  The promoter sequence was also scanned for the restriction enzymes in all assembly standards to determine if it could be use for iGEM and it was determined to be compatible with all the standards.     
−
 
+
−
pTEF was then isolated and compared with another strong constitutive promoter, glyceraldehydes-3-phosphate dehydrogenase promoter (pGAP).  According to research done on this promoter it has been determine that when compared to pGAP, pTEF was shown to work well in high glucose environments and carbon-limited conditions, therefore leading to similar or even greater expression levels of recombinant protein.  It was also determined that pTEF does not require an inducer, since it is a constitutive promoter.  pTEF can also be used in the mass production of heterologuous proteins.  From the research done on this promoter it was concluded that pTEF can provide more choices on production conditions and easier methods for expression than pGAP.  Therefore, pTEF would be a great alternative for expressing foreign genes in Pichia pastoris for industrial application.  This promoter sequence can be isolated from yeast Pichia pastoris.  The promoter sequence was also scanned for the restriction enzymes in all assembly standards to determine if it could be use for iGEM and it was determined to be compatible with all the standards.     
+
−
 
+
−
 
+
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 17:58, 27 October 2010

pTEF ( Translation elongation factor promoter)

pTEF The TEF1- α gene is comprised of 1.380 kB and encodes a polypeptide of 459 amino acids and a molecular weight of 50.1 kDa. The final product is the soluble protein Translational Elongation Factor. This protein has been determined to be one of the most abundant proteins in eukaryotic cells and known to be associated with cell growth. Recognizing the high level of the protein present in the cell, it was predicted that TEF1- α is under the control of a strong promoter. It was discovered that the translational elongation factor is under the control of strong constitutive promoter pTEF.pTEF was then isolated and compared with another strong constitutive promoter, glyceraldehydes-3-phosphate dehydrogenase promoter (pGAP). According to research done on this promoter it has been determine that when compared to pGAP, pTEF was shown to work well in high glucose environments and carbon-limited conditions, therefore leading to similar or even greater expression levels of recombinant protein. It was also determined that pTEF does not require an inducer, since it is a constitutive promoter. pTEF can also be used in the mass production of heterologuous proteins. From the research done on this promoter it was concluded that pTEF can provide more choices on production conditions and easier methods for expression than pGAP. Therefore, pTEF would be a great alternative for expressing foreign genes in Pichia pastoris for industrial application. This promoter sequence can be isolated from yeast Pichia pastoris. The promoter sequence was also scanned for the restriction enzymes in all assembly standards to determine if it could be use for iGEM and it was determined to be compatible with all the standards.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]