Difference between revisions of "Part:BBa J33204:Experience"
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<I>Imperial College iGEM 2010</I>
 
<I>Imperial College iGEM 2010</I>
 
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*The enzymatic reaction catalysed by C2,3O is an ideal output signal for our engineered bacterial detector and it can also serve as a very useful reporter gene.
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*Catechol, the substrate of C2,3O, is colourless. However within seconds of its addition, the colonies/liquid cultures of XylE-expressing cells become yellow, indicating production of a product which absorbs light in the visible spectrum
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*The spectra (figure 1) showed that in XylE transformed cells, a broad peak appears at about '''380nm'''. The absorbance at this particular wavelength is by the product of the C2,3O reaction which is called 2-hydroxymuconic semialdehyde and is what causes the yellow output.
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|Spectra of cultures of cells expressing the XylE and cells negative for the XylE gene. Note the broad peak in the spectra of Xyle transformed cells, which is centered around 380nm.
 
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Revision as of 17:29, 27 October 2010

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Imperial College iGEM 2010

  • The enzymatic reaction catalysed by C2,3O is an ideal output signal for our engineered bacterial detector and it can also serve as a very useful reporter gene.
  • Catechol, the substrate of C2,3O, is colourless. However within seconds of its addition, the colonies/liquid cultures of XylE-expressing cells become yellow, indicating production of a product which absorbs light in the visible spectrum
  • The spectra (figure 1) showed that in XylE transformed cells, a broad peak appears at about 380nm. The absorbance at this particular wavelength is by the product of the C2,3O reaction which is called 2-hydroxymuconic semialdehyde and is what causes the yellow output.
[1]
Spectra of cultures of cells expressing the XylE and cells negative for the XylE gene. Note the broad peak in the spectra of Xyle transformed cells, which is centered around 380nm.

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