Difference between revisions of "Part:BBa K395307"
 
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<partinfo>BBa_K395307 short</partinfo>
 
<partinfo>BBa_K395307 short</partinfo>
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This part was rebuilt in order to measure its strength on the low copy plasmid, pSB3K3.
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The transcriptional activity of of R0082(ompC-WT promoter)was measured through the GFP expression.[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about P''ompC'' series]
  
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Measuring the activity of R0082(ompC-WT promoter) through the expression of GFP
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[[Image:Tokyotech_ompc_graph.jpg|thumb|center|400px|Figure 1. Induction of new'' OmpC'' series in high osmolarity medium at 4 hours.<br/>This work is done by Taichi Nakamura & Thiprampai THAMAMONGOOD <br/>Tokyo Tech iGEM2010]]
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The difference from R0082 data is plasmid. We assayed this BBa_K395307 with backbone pSB3K3.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 17:15, 27 October 2010

PompC(WT) with GFP reporter (R0082+E0240)
This part was rebuilt in order to measure its strength on the low copy plasmid, pSB3K3. The transcriptional activity of of R0082(ompC-WT promoter)was measured through the GFP expression.[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/New_Series_of_PompC ...see more about PompC series]

Figure 1. Induction of new OmpC series in high osmolarity medium at 4 hours.
This work is done by Taichi Nakamura & Thiprampai THAMAMONGOOD
Tokyo Tech iGEM2010

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 779