Difference between revisions of "Part:BBa K389014"
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This Device can be used for characterization and screening of mutated VirA receptors. | This Device can be used for characterization and screening of mutated VirA receptors. | ||
− | For a working VirA/G-receptor system, you also need the ''virA'' BioBrick <partinfo>K389001</partinfo>. You can find the complete working system here: <partinfo> | + | For a working VirA/G-receptor system, you also need the ''virA'' BioBrick <partinfo>K389001</partinfo>. You can find the complete working system here: <partinfo>K389016</partinfo> |
Revision as of 17:03, 27 October 2010
VirA/G screening system test
This BioBrick is made to test our virA screening system and the strategy for directed evolution, respectively. It contains an unmutated virA under the control of a constitutive promoter and the screening device BBa_K389011.
Usage and Biology
This Device can be used for characterization and screening of mutated VirA receptors.
For a working VirA/G-receptor system, you also need the virA BioBrick BBa_K389001. You can find the complete working system here: BBa_K389016
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 647
Illegal NheI site found at 2581
Illegal NheI site found at 2604 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1632
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 1768