Difference between revisions of "Part:BBa K322210"
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Using the BRIDGE protocol, the markers are removed every time you insert a new gene, so they can be used again and again indefinitely. You could essentially replace the entire genome with biobricked versions. | Using the BRIDGE protocol, the markers are removed every time you insert a new gene, so they can be used again and again indefinitely. You could essentially replace the entire genome with biobricked versions. | ||
| − | Notes: has complete suffix but prefix lacks NotI site. | + | Notes: This biobrick was submitted in compliance with RFC 54, which was jointly developed by Osaka and us. It has complete suffix but prefix lacks NotI site. It is still completely compatible with RFC10. |
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 16:54, 27 October 2010
Chloramphenicol acetyltransferase (cat)
cat is an antibiotic resistance gene, used in the first step of BRIDGE which requires the deletion of existing DNA (probably a non-coding piece or a non-essential gene) to select for cells which have taken up the construct by growing them on the relevant antibiotic.
Using the BRIDGE protocol, the markers are removed every time you insert a new gene, so they can be used again and again indefinitely. You could essentially replace the entire genome with biobricked versions.
Notes: This biobrick was submitted in compliance with RFC 54, which was jointly developed by Osaka and us. It has complete suffix but prefix lacks NotI site. It is still completely compatible with RFC10.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]