Difference between revisions of "Part:BBa K320005:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
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===Applications of BBa_K320005===
 
===Applications of BBa_K320005===
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We used this BioBrick to characterize the strong promoter we want to use in Asaia <partinfo>BBa_K320002</partinfo>. By measuring the CFP fluorescence over time, we can infer the activity of the promoter. To check whether the presence of CFP or the construct reduces the growth of the cells, we measured the optical density and compared it to a WT-E.coli.<br />
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Both measurements were done simultaneously in the ''E. coli'' strain DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.<br />
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The controls for the autofluorescence and the growth curve were done with a WT strain of DH5a.
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[[Image:800px-EPFL_OD_prom.png|800px|center]]
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[[Image:PromOD.png|800px|center]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 16:37, 27 October 2010

Applications of BBa_K320005

We used this BioBrick to characterize the strong promoter we want to use in Asaia BBa_K320002. By measuring the CFP fluorescence over time, we can infer the activity of the promoter. To check whether the presence of CFP or the construct reduces the growth of the cells, we measured the optical density and compared it to a WT-E.coli.
Both measurements were done simultaneously in the E. coli strain DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.
The controls for the autofluorescence and the growth curve were done with a WT strain of DH5a.


800px-EPFL OD prom.png
PromOD.png

User Reviews

UNIQb864e3edb1a7142a-partinfo-00000001-QINU UNIQb864e3edb1a7142a-partinfo-00000002-QINU