Difference between revisions of "Part:BBa K320005"
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This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(<partinfo>BBa_K320002</partinfo>) and a CFP biobrick (<partinfo>BBa_E0020</partinfo>). | This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(<partinfo>BBa_K320002</partinfo>) and a CFP biobrick (<partinfo>BBa_E0020</partinfo>). | ||
− | This BioBrick was principally build to test the constitutive promoter we syntetise (<partinfo>BBa_K320002</partinfo>) | + | This BioBrick was principally build to test the constitutive promoter we syntetise (<partinfo>BBa_K320002</partinfo>). |
+ | |||
+ | We only manage to caracterize it in E.Coli (see the experiement page). Further we will caractreize it in Asaia to continue building gear for our new chassis (http://2010.igem.org/Team:EPF_Lausanne/Project_asaia ''Asaia''). | ||
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Revision as of 16:14, 27 October 2010
Constitutive expression of CFP
This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(BBa_K320002) and a CFP biobrick (BBa_E0020).
This BioBrick was principally build to test the constitutive promoter we syntetise (BBa_K320002).
We only manage to caracterize it in E.Coli (see the experiement page). Further we will caractreize it in Asaia to continue building gear for our new chassis (http://2010.igem.org/Team:EPF_Lausanne/Project_asaia Asaia).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 62
Illegal NheI site found at 146 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]