Difference between revisions of "Part:BBa K320005"

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This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(<partinfo>BBa_K320002</partinfo>) and a CFP biobrick (<partinfo>BBa_E0020</partinfo>).
 
This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(<partinfo>BBa_K320002</partinfo>) and a CFP biobrick (<partinfo>BBa_E0020</partinfo>).
  
This BioBrick was principally build to test the constitutive promoter we syntetise (<partinfo>BBa_K320002</partinfo>) and will use in Asaia.  
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This BioBrick was principally build to test the constitutive promoter we syntetise (<partinfo>BBa_K320002</partinfo>).
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We only manage to caracterize it in E.Coli (see the experiement page). Further we will caractreize it in Asaia to continue building gear for our new chassis (http://2010.igem.org/Team:EPF_Lausanne/Project_asaia ''Asaia'').
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:14, 27 October 2010

Constitutive expression of CFP

This BioBrick espresses CFP in a constitutive way. It was assembled from a strong constitutive promoter with a RBS(BBa_K320002) and a CFP biobrick (BBa_E0020).

This BioBrick was principally build to test the constitutive promoter we syntetise (BBa_K320002).

We only manage to caracterize it in E.Coli (see the experiement page). Further we will caractreize it in Asaia to continue building gear for our new chassis (http://2010.igem.org/Team:EPF_Lausanne/Project_asaia Asaia).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
    Illegal NheI site found at 146
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]