Difference between revisions of "Part:BBa K320006:Design"

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===Design Notes===
 
===Design Notes===
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We ligate two BioBricks quoted below.  
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We ligated the two BioBricks quoted below.  
  
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The first BioBricks (<partinfo>BBa_K320001</partinfo>) was intial in the <partinfo>pSB1C3</partinfo> backbone vector. We opened it with the restriction enzyme SpeI and PstI.  
+
The first BioBrick (<partinfo>BBa_K320001</partinfo>) was initially in the <partinfo>pSB1C3</partinfo> backbone vector. We opened it with the restriction enzymes SpeI and PstI.  
  
−
The second part (<partinfo>BBa-K320002</partinfo>) was also stored in the <partinfo>pSB1C3</partinfo> backbone vector. This one we digested with Xba and PstI.
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The second part (<partinfo>BBa-K320002</partinfo>) was also stored in the <partinfo>pSB1C3</partinfo> backbone vector. We digested this one with Xba and PstI.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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# [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes]

Latest revision as of 16:07, 27 October 2010

Constitutive expression of Immunotoxin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 883
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We ligated the two BioBricks quoted below.

The first BioBrick (BBa_K320001) was initially in the pSB1C3 backbone vector. We opened it with the restriction enzymes SpeI and PstI.

The second part (BBa_K320002) was also stored in the pSB1C3 backbone vector. We digested this one with Xba and PstI.

Source

The Two BioBricks we used are BBa_K320001 and BBa_K320002.

References

  1. [http://linkinghub.elsevier.com/retrieve/pii/S016668510000387X Bacteria expressing single-chain immunotoxin inhibit malaria parasite development in mosquitoes]