Difference between revisions of "Part:BBa K299817"

Latest revision as of 16:00, 27 October 2010

This part is licensed under
Creative BioCommons

control construct - GFP under J23102

Green Fluorescent Protein is a noninvasive fluorescent marker for gene expression, protein localisation and intracellular protein targeting. Originally from Aequorea victoria.

Authors:

Cloned by Joanna Leszczyńska.

Work supervised by Michał Lower.

Construct design

The part consists of J23102 promoter and B0032 RBS ligated to GFP. Double terminator (B0010+B0012) guarantees the stability of polycistronic RNA as a product of transcription. The construct is a control device used in testing of the Invasiveness Operon (BBa_K299813 and BBa_K299815) To find out more about it's background and design click here.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 708

@@ Line 12: / Line 12: @@
 <p><h3>Construct design</h3>
 The part consists of J23102 promoter and B0032 RBS ligated to GFP. Double terminator (B0010+B0012) guarantees the stability of polycistronic RNA as a product of transcription. The construct is a control device used in testing of the Invasiveness Operon (<html><a href="https://parts.igem.org/wiki/index.php/Part:BBa_K299813">BBa_K299813</a> and <a href="https://parts.igem.org/wiki/index.php/Part:BBa_K299815">BBa_K299815</a></html>) To find out more about it's background and design <html><a href="http://2010.igem.org/Team:Warsaw/Stage3">click here.</a></html></p>
 <!-- Add more about the biology of this part here