Difference between revisions of "Part:BBa K299817"
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| + | <p><b>Green Fluorescent Protein</b> is a noninvasive fluorescent marker for gene expression, protein localisation and intracellular protein targeting. Originally from <i>Aequorea victoria.</i></p> | ||
| + | <p><h3>Authors:</h3> | ||
| + | Cloned by Joanna Leszczyńska. | ||
| + | Work supervised by Michał Lower.</p> | ||
| + | <p><h3>Construct design</h3> | ||
| + | The part consists of J23102 promoter and B0032 RBS ligated to GFP. Double terminator (B0010+B0012) guarantees the stability of polycistronic RNA as a product of transcription. The construct is a control device used in testing of the Invasiveness Operon (<html><a href="https://parts.igem.org/wiki/index.php/Part:BBa_K299813">BBa_K299813</a> and <a href="https://parts.igem.org/wiki/index.php/Part:BBa_K299815">BBa_K299815</a></html>) To find out more about it's background and design <html><a href="http://2010.igem.org/Team:Warsaw/Stage3">click here.</a></html></p> | ||
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Latest revision as of 16:00, 27 October 2010
This part is licensed under
Creative BioCommons
control construct - GFP under J23102
Green Fluorescent Protein is a noninvasive fluorescent marker for gene expression, protein localisation and intracellular protein targeting. Originally from Aequorea victoria.
Authors:
Cloned by Joanna Leszczyńska.
Work supervised by Michał Lower.Construct design
The part consists of J23102 promoter and B0032 RBS ligated to GFP. Double terminator (B0010+B0012) guarantees the stability of polycistronic RNA as a product of transcription. The construct is a control device used in testing of the Invasiveness Operon (BBa_K299813 and BBa_K299815) To find out more about it's background and design click here.Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 708