Difference between revisions of "Part:BBa K299817"

 
 
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<partinfo>BBa_K299817 short</partinfo>
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{{BioCommons}}
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<h2><partinfo>BBa_K299817 short</partinfo></h2>
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<p><b>Green Fluorescent Protein</b> is a noninvasive fluorescent marker for gene expression, protein localisation and intracellular protein targeting. Originally from <i>Aequorea victoria.</i></p>
  
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<p><h3>Authors:</h3>
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Cloned by Joanna Leszczyńska.
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Work supervised by Michał Lower.</p>
  
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<p><h3>Construct design</h3>
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The part consists of J23102 promoter and B0032 RBS ligated to GFP. Double terminator (B0010+B0012) guarantees the stability of polycistronic RNA as a product of transcription. The construct is a control device used in testing of the Invasiveness Operon (<html><a href="https://parts.igem.org/wiki/index.php/Part:BBa_K299813">BBa_K299813</a> and <a href="https://parts.igem.org/wiki/index.php/Part:BBa_K299815">BBa_K299815</a></html>) To find out more about it's background and design <html><a href="http://2010.igem.org/Team:Warsaw/Stage3">click here.</a></html></p>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:00, 27 October 2010

This part is licensed under
Creative BioCommons

control construct - GFP under J23102


Green Fluorescent Protein is a noninvasive fluorescent marker for gene expression, protein localisation and intracellular protein targeting. Originally from Aequorea victoria.

Authors:

Cloned by Joanna Leszczyńska.

Work supervised by Michał Lower.

Construct design

The part consists of J23102 promoter and B0032 RBS ligated to GFP. Double terminator (B0010+B0012) guarantees the stability of polycistronic RNA as a product of transcription. The construct is a control device used in testing of the Invasiveness Operon (BBa_K299813 and BBa_K299815) To find out more about it's background and design click here.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 708