Difference between revisions of "Part:BBa K300091"

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<partinfo>BBa_K300091 short</partinfo>
 
<partinfo>BBa_K300091 short</partinfo>
  
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This construct was used to test the possibility to induce the synthesis of fusion proteins composed by phasin (<partinfo>BBa_K300002</partinfo>), intein (<partinfo>BBa_K300004</partinfo>), linkers (<partinfo>BBa_K105012</partinfo>) and GFP reporter (<partinfo>BBa_K300005</partinfo>). With this BioBrick was also tested the right folding of the recombinant proteins
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This construct was used to test the possibility of inducing the synthesis of fusion proteins composed by phasin (<partinfo>BBa_K300002</partinfo>), intein (<partinfo>BBa_K300004</partinfo>) and linker (<partinfo>BBa_K105012</partinfo>). With this BioBrick the right folding of the recombinant protein was also tested, by measuring the GFP reporter (<partinfo>BBa_K125500</partinfo>).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:27, 27 October 2010

3OC6HSL inducible generator for BBa_K300095

This construct was used to test the possibility of inducing the synthesis of fusion proteins composed by phasin (BBa_K300002), intein (BBa_K300004) and linker (BBa_K105012). With this BioBrick the right folding of the recombinant protein was also tested, by measuring the GFP reporter (BBa_K125500).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 2859
    Illegal SapI.rc site found at 2165