Difference between revisions of "Part:BBa K300091"

 
 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K300091 short</partinfo>
 
<partinfo>BBa_K300091 short</partinfo>
  
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This construct was used to test the possibility to induce the synthesis of fusion proteins composed by phasins, intein and GFP reporter. With this BioBrick was also tested the right folding of the recombinant proteins
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This construct was used to test the possibility of inducing the synthesis of fusion proteins composed by phasin (<partinfo>BBa_K300002</partinfo>), intein (<partinfo>BBa_K300004</partinfo>) and linker (<partinfo>BBa_K105012</partinfo>). With this BioBrick the right folding of the recombinant protein was also tested, by measuring the GFP reporter (<partinfo>BBa_K125500</partinfo>).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:27, 27 October 2010

3OC6HSL inducible generator for BBa_K300095

This construct was used to test the possibility of inducing the synthesis of fusion proteins composed by phasin (BBa_K300002), intein (BBa_K300004) and linker (BBa_K105012). With this BioBrick the right folding of the recombinant protein was also tested, by measuring the GFP reporter (BBa_K125500).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1004
    Illegal BsaI.rc site found at 2859
    Illegal SapI.rc site found at 2165