Difference between revisions of "Part:BBa K398029:Experience"

(→‎Applications of BBa_K398029)
(→‎Applications of BBa_K398029)
 
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*Strain 030A: Escherichia coli K12 carrying the part [https://parts.igem.org/Part:BBa_K398030 BBa_K398030] in the plasmid pSB1A2.
 
*Strain 030A: Escherichia coli K12 carrying the part [https://parts.igem.org/Part:BBa_K398030 BBa_K398030] in the plasmid pSB1A2.
 
*Positive control: ''Pseudomonas putida'' growing on a culture with M9 medium using octane (1% v/v) as sole carbon source.
 
*Positive control: ''Pseudomonas putida'' growing on a culture with M9 medium using octane (1% v/v) as sole carbon source.
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The expression profiles shows that this part is not being overexpressed in ''E. coli'' as we planned.
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[[Image:TUDelftALDH_exp.jpg|600px|thumb|center|Expression profile of different recombinant ''E. coli'' strains, among them 029A. The expected size for ALDH is around 53 kDa.]]
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 15:26, 27 October 2010

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K398029

  • iGEM TU Delft 2010

We characterized the parts: BBa_K398029 and BBa_K398030 Check [http://2010.igem.org/Team:TU_Delft#page=Project/alkane-degradation/results our website] of results in order to know about our experience with this part!.

Comparison of ALDH activities in the different cell extracts tested in our study.

Notes:

  • Negative control: Escherichia coli K12 carrying the part BBa_J13002 in the plasmid pSB1A2.
  • Strain 029A: Escherichia coli K12 carrying the part BBa_K398029 in the plasmid pSB1A2.
  • Strain 030A: Escherichia coli K12 carrying the part BBa_K398030 in the plasmid pSB1A2.
  • Positive control: Pseudomonas putida growing on a culture with M9 medium using octane (1% v/v) as sole carbon source.

The expression profiles shows that this part is not being overexpressed in E. coli as we planned.

Expression profile of different recombinant E. coli strains, among them 029A. The expected size for ALDH is around 53 kDa.

User Reviews

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