Difference between revisions of "Part:BBa K398406"

(Introduction)
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==Introduction==
 
==Introduction==
 
The effect of organic solvents to organisms can be detrimental: they can accumulate in the cell membrane changing the composition, thus affecting its physiological function. BBa_K398406 produces prefoldin, a protein from ''Pyrococcus horikoshii OT3''. Prefoldin is a part of a widely spread group of proteins: chaperones. These proteins have the property of helping during the protein folding process, and are related also to thermal shock resistance. The prefoldin constists of two subunits: phPFDα and phPFDβ (Figure 1). These proteins increase the tolerance to alkanes by acting as chaperones, which correct protein misfolding /unfolding in the presence of alkanes.
 
The effect of organic solvents to organisms can be detrimental: they can accumulate in the cell membrane changing the composition, thus affecting its physiological function. BBa_K398406 produces prefoldin, a protein from ''Pyrococcus horikoshii OT3''. Prefoldin is a part of a widely spread group of proteins: chaperones. These proteins have the property of helping during the protein folding process, and are related also to thermal shock resistance. The prefoldin constists of two subunits: phPFDα and phPFDβ (Figure 1). These proteins increase the tolerance to alkanes by acting as chaperones, which correct protein misfolding /unfolding in the presence of alkanes.
[[<html><img src="https://static.igem.org/mediawiki/2010/2/2f/TU_Delft_SaltModel.jpg"></html>|500px|center|thumb|figure 1: Model of the Solvent tolerance]]
+
[[<html><img src="https://static.igem.org/mediawiki/2010/2/2f/TU_Delft_SaltModel.jpg" width="500"></html>|500px|center|thumb|figure 1: Model of the Solvent tolerance]]
  
 
The [http://2010.igem.org/Team:TU_Delft#page=Project/tolerance characterization of BBa_K398406] has been described on the TU Delft iGEM Team 2010 wiki.
 
The [http://2010.igem.org/Team:TU_Delft#page=Project/tolerance characterization of BBa_K398406] has been described on the TU Delft iGEM Team 2010 wiki.

Revision as of 15:25, 27 October 2010

Solvent tolerance cluster

BBa_K398406 enables E.coli of working in two-phase systems with resistance to high concentrations of hydrophobic molecules in the membrane. A growth rate increase of 62% in comparison to a standard E.coli was achieved at concentrations of 8% (v/v) n-hexane.

File:Protein 2ZDI.gif
Figure 1 - Proteins [http://www.rcsb.org/pdb/explore/explore.do?pdbId=2ZDI phPFDα and phPFDβ]

Introduction

The effect of organic solvents to organisms can be detrimental: they can accumulate in the cell membrane changing the composition, thus affecting its physiological function. BBa_K398406 produces prefoldin, a protein from Pyrococcus horikoshii OT3. Prefoldin is a part of a widely spread group of proteins: chaperones. These proteins have the property of helping during the protein folding process, and are related also to thermal shock resistance. The prefoldin constists of two subunits: phPFDα and phPFDβ (Figure 1). These proteins increase the tolerance to alkanes by acting as chaperones, which correct protein misfolding /unfolding in the presence of alkanes. [[|500px|center|thumb|figure 1: Model of the Solvent tolerance]]

The [http://2010.igem.org/Team:TU_Delft#page=Project/tolerance characterization of BBa_K398406] has been described on the TU Delft iGEM Team 2010 wiki.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 37
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 37
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 37
  • 1000
    COMPATIBLE WITH RFC[1000]