Difference between revisions of "Part:BBa K300090"

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<partinfo>BBa_K300090 short</partinfo>
 
<partinfo>BBa_K300090 short</partinfo>
  
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This part was built to quantify the affinity and binding activity of a head (<partinfo>BBa_K300002</partinfo>) and internal domain (<partinfo>BBa_K300003</partinfo>) phasin separed by by a flexible protein domain linker (<partinfo>BBa_K105012</partinfo>) to polyhydroxyalkanoates (PHAs) granules. It can be also be used as a device for low cost purification of recombinant proteins.
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This part was built to quantify the affinity and binding activity of a head (<partinfo>BBa_K300002</partinfo>) and internal domain (<partinfo>BBa_K300003</partinfo>) phasin separed by a flexible protein domain linker (<partinfo>BBa_K105012</partinfo>) to polyhydroxyalkanoates (PHAs) granules. With this BioBrick the right folding of the recombinant protein was also tested, by measuring the GFP reporter (<partinfo>BBa_K125500</partinfo>).
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 15:22, 27 October 2010

Measurement system for BBa_K300094

This part was built to quantify the affinity and binding activity of a head (BBa_K300002) and internal domain (BBa_K300003) phasin separed by a flexible protein domain linker (BBa_K105012) to polyhydroxyalkanoates (PHAs) granules. With this BioBrick the right folding of the recombinant protein was also tested, by measuring the GFP reporter (BBa_K125500).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1921