Difference between revisions of "Part:BBa K310007:Design"
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<partinfo>BBa_K310007 short</partinfo> | <partinfo>BBa_K310007 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | Site directed mutagenesis was performed in the coding region of ArsR due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT. | + | Site directed mutagenesis was performed in the coding region of ArsR at nucleotide 139 due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT. |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | This paper was particularly helpful when working with the Ars Operon and Arsenic parts within E. coli: [http://aem.asm.org/cgi/reprint/70/8/4582 Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR] | ||
Latest revision as of 14:47, 27 October 2010
ArsR repressor of the Ars Operon in E. coli K-12 MG1655
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 138
Design Notes
Site directed mutagenesis was performed in the coding region of ArsR at nucleotide 139 due to the presence of XbaI site. This changed the coding sequence AGATCT to AGGTCT.
Source
E. coli K-12 MG1655 genome
References
This paper was particularly helpful when working with the Ars Operon and Arsenic parts within E. coli: [http://aem.asm.org/cgi/reprint/70/8/4582 Enhanced Arsenic Accumulation in Engineered Bacterial Cells Expressing ArsR]