Difference between revisions of "Part:BBa K323110"

Revision as of 13:14, 27 October 2010

pBAD_BsaI_DTER

This part is composited from the pBAD promoter (Part:BBa_I0500), a double terminator (Part:BBa_B0015) and a BsaI restriction site inbetween. The BsaI restriction endonuclease cuts the DNA outside of its recognition site. BsaI restriction site (clone in site) is designed in such a way that any BioBrick part, cut with XbaI and NotI enzymes can be ligated into this vector cut with the BsaI enzyme.

Therefore, the purpose of this part is cloning any BioBrick protein coding sequence between a promoter and a terminator in only one ligation instead of two.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1145
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 980
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1260
Illegal BsaI.rc site found at 1236
Illegal SapI site found at 962

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K323110 short</partinfo>
-This part is composited from the pBAD promoter ([[Part:BBa_I0500]]), a double terminator ([[Part:BBa_B0015]]) and a ''Bsa''I restriction site inbetween. The ''Bsa''I restriction endonuclease cuts the DNA outside of its recognition site, and the ''Bsa''I restriction site (clone in site) is designed in such a way that any BioBrick part, cut with ''Xba''I and ''Not''I enzymes can be ligated into this vector cut with the ''Bsa''I enzyme.
+This part is composited from the pBAD promoter ([[Part:BBa_I0500]]), a double terminator ([[Part:BBa_B0015]]) and a ''Bsa''I restriction site inbetween. The ''Bsa''I restriction endonuclease cuts the DNA outside of its recognition site. ''Bsa''I restriction site (clone in site) is designed in such a way that any BioBrick part, cut with ''Xba''I and ''Not''I enzymes can be ligated into this vector cut with the ''Bsa''I enzyme.
 Therefore, the purpose of this part is cloning any BioBrick protein coding sequence between a promoter and a terminator in only one ligation instead of two.