Constitutive expression of P25 and P28 (driven by K320002) with an origin for Asaia and E. coli and a resiatance to Tetracycline .
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Constitutive expression of P25 and P28 (driven by <partinfo>K320002</partinfo>), replication origin for Asaia and ''E. coli'' and a resistance to Tetracycline .
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This BioBricks makes the bacteria resitant to Tetracycline, contains an origin of replication for Asaia/E. coli and drives constitutive expression of the proteins P28 and P25.
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The ookinete (plasmodium state in mosquito's gut) has been intensively studied by scientists, looking for an ideal transmission-blocking vaccine target. IGEM EPFL team's interest goes toward the ookinete surface membrane proteins P25 and P28. In fact, it has been shown that the binding of specific antibodies to these surface proteins was fatal to more than 99% of the parasites reference. Indeed, P25 and P28 seem to be essential to the ookinete survival, the crossing of the midgut epithelium and its transformation into an oocyst.
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The team decided to design Asaia to express a soluble form of these 2 P-proteins. It could seems contra-intuitive to provide more of these proteins (by expression in Asaia) that are very important for the parasite survival, migration, and development. But our hypothesis is that adding a lot of these proteins in a soluble form will compete with the parasite surface protein and prevent the interaction necessary to its transmission. Because the functions of P25 and P28 are redundant, both proteins need to be inhibited or outcompeted to efficiently block the malaria transmission.
Latest revision as of 13:08, 27 October 2010
S + P25 + P28 + Ori + Tet
Constitutive expression of P25 and P28 (driven by BBa_K320002), replication origin for Asaia and E. coli and a resistance to Tetracycline .
Sequence and Features
Assembly Compatibility:
10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 62 Illegal NheI site found at 827
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2699 Illegal AgeI site found at 2859
