Difference between revisions of "Part:BBa K404152"

 
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VP1up protein is a derivate of Vp1 protein, which contains several point mutations that lead to significant decrease in enzymatic activity of phospholipase. Phospholipase is located on N terminus in VP1  [Canaan et al., 2004; Zadori et al., 2001], which is required for virus internalisation [Girod et al., 2002]
  
 
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<partinfo>BBa_K404152 parameters</partinfo>
 
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<h3>References</h3>
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<b>Canaan</b>, 2004. Interfacial enzymology of parvovirus phospholipases A2. The Journal of Biological Chemistry, 279(15), pp.14502-14508. <br />
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<b>Girod, A.</b>, 2002. The VP1 capsid protein of adeno-associated virus type 2 is carrying a phospholipase A2 domain required for virus infectivity. The Journal of general virology, 83(Pt 5), pp.973-978. Available at: http://www.ncbi.nlm.nih.gov/pubmed/11961250.<br />
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<b>Zadori, Z </b>, 2001. A viral phospholipase A2 is required for parvovirus infectivity. Developmental Cell, 1(2), pp.291-302. Available at: http://www.ncbi.nlm.nih.gov/pubmed/11702787. <br />

Revision as of 12:20, 27 October 2010

[AAV2]-VP1up

VP1up protein is a derivate of Vp1 protein, which contains several point mutations that lead to significant decrease in enzymatic activity of phospholipase. Phospholipase is located on N terminus in VP1 [Canaan et al., 2004; Zadori et al., 2001], which is required for virus internalisation [Girod et al., 2002]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 28
    Illegal XhoI site found at 214
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

Canaan, 2004. Interfacial enzymology of parvovirus phospholipases A2. The Journal of Biological Chemistry, 279(15), pp.14502-14508.
Girod, A., 2002. The VP1 capsid protein of adeno-associated virus type 2 is carrying a phospholipase A2 domain required for virus infectivity. The Journal of general virology, 83(Pt 5), pp.973-978. Available at: http://www.ncbi.nlm.nih.gov/pubmed/11961250.
Zadori, Z , 2001. A viral phospholipase A2 is required for parvovirus infectivity. Developmental Cell, 1(2), pp.291-302. Available at: http://www.ncbi.nlm.nih.gov/pubmed/11702787.