Difference between revisions of "Part:BBa K336053"
| (One intermediate revision by the same user not shown) | |||
| Line 3: | Line 3: | ||
To obtain large amount of protein, over expression system was designed. | To obtain large amount of protein, over expression system was designed. | ||
| − | Expression of T7 polymerase | + | Expression of T7 polymerase is induced by IPTG. |
Add IPTG when you need, you obtain target of protein at appropriate　phase. | Add IPTG when you need, you obtain target of protein at appropriate　phase. | ||
| − | |||
| − | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 11:51, 27 October 2010
Over expression system (T7 polymerase induced by lac promoter)
To obtain large amount of protein, over expression system was designed. Expression of T7 polymerase is induced by IPTG. Add IPTG when you need, you obtain target of protein at appropriate phase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]