Difference between revisions of "Part:BBa K395602"
Line 6: | Line 6: | ||
− | [[Image:K395602.png|600px|center| | + | [[Image:K395602.png|600px|center|thumb|Apple fragrance expression device]] |
+ | |||
+ | [[Image:Tokyotech apple fragrance GC.png|600px|center|thumb|Gas chromatography analysis of apple fragrance expression device]] | ||
+ | (1) MpAAT1 + BuOH, (2) No MpAAT1 + BuOAc, (3) No MpAAT1 + BuOH, (4) MpAAT1 + 2-MeBuOH, (5) No MpAAT1 + 2-MeBuOAc, (6) No MpAAT1 + 2-MeBuOH, (7) MpAAT1 - 2-MeBuOH. | ||
Revision as of 11:50, 27 October 2010
Apple fragrance generator
The apple fragrance generator (BBa_K395602) catalyzes the conversion of 2-methylbutanol and butanol to the odor 2-methybutyl acetate and butyl acetate that has a apple fragrance. The biosynthetic device is composed of two devices:fragrance enzyme generator (BBa_K395601) and a T7 terminator (BBa_K395600).
(1) MpAAT1 + BuOH, (2) No MpAAT1 + BuOAc, (3) No MpAAT1 + BuOH, (4) MpAAT1 + 2-MeBuOH, (5) No MpAAT1 + 2-MeBuOAc, (6) No MpAAT1 + 2-MeBuOH, (7) MpAAT1 - 2-MeBuOH.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1765
Illegal NotI site found at 1666 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 195
Illegal BamHI site found at 1635
Illegal XhoI site found at 1675 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 205
Illegal AgeI site found at 1419 - 1000COMPATIBLE WITH RFC[1000]