Difference between revisions of "Part:BBa I13453"

m
Line 11: Line 11:
 
See this OpenWetWare article on [http://openwetware.org/wiki/Titratable_control_of_pBAD_and_lac_promoters_in_individual_E._coli_cells#pBAD_promotersOpenWetWare pBAD and lac promoters] for additional usage and biology information
 
See this OpenWetWare article on [http://openwetware.org/wiki/Titratable_control_of_pBAD_and_lac_promoters_in_individual_E._coli_cells#pBAD_promotersOpenWetWare pBAD and lac promoters] for additional usage and biology information
  
===Characterization===
+
 
For the 2009 iGEM competition, British_Columbia characterized <partinfo>I13453</partinfo> in the context of a [https://parts.igem.org/PBAD_Promoter_Family pBAD promoter family]. For the results of this characterization, see [[Part:BBa_I13453:British_Columbia|here]].
+
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_I13453 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_I13453 SequenceAndFeatures</partinfo>
  
 +
===Characterization===
 +
For the 2009 iGEM competition, British_Columbia characterized <partinfo>I13453</partinfo> in the context of a [https://parts.igem.org/PBAD_Promoter_Family pBAD promoter family]. For the results of this characterization, see [[Part:BBa_I13453:British_Columbia|here]].
 +
 +
For the 2010 iGEM competition, Tec-Monterrey characterized <partinfo>I13453</partinfo> again, using a construct of GFP reporter after the pBad promoter. The transfer function was modeled with a Hill equation.
 +
<!-- -->
 +
[[Image:pbadwt.png|center|frame|Figure 1. Transfer function of <partinfo>BBa_I13453</partinfo>. Points represent individual measurements. The line is of a Hill equation fitted to our data.]]
 +
<center>
 +
(d[GFP]/dt)/OD<sub>600</sub> = C+A*X<sup>n</sup>/(X<sup>n</sup>+K<sup>n</sup>)
 +
{|{{Table}}
 +
!Experiment
 +
!Characteristic
 +
!Value
 +
|-
 +
|rowspan="4"|[[#Transfer Function|'''Transfer Function''']]
 +
|''Basal rate (C)''
 +
|1 d[GFP]/dt
 +
|-
 +
|''Gain (A)''
 +
|36 d[GFP]/dt
 +
|-
 +
|''Hill coefficient (n)''
 +
|2.16
 +
|-
 +
|''Switch Point (K)''
 +
|2.8 [ara] (µM)
 +
 +
|-
 +
|}
 +
</center>
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 11:45, 27 October 2010

Pbad promoter

PBad promoter from I0500 without AraC.


Usage and Biology

Has been used as a second promoter in a system containing BBa_I0500 (PBad+AraC). In this system, it showed behavior qualitatively indistinguishable from the BBa_I0500 copy of PBad. Has not been tested independent of AraC. A second part, BBa_I13458, should allow decoupling of PBad and AraC.

See this OpenWetWare article on [http://openwetware.org/wiki/Titratable_control_of_pBAD_and_lac_promoters_in_individual_E._coli_cells#pBAD_promotersOpenWetWare pBAD and lac promoters] for additional usage and biology information


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterization

For the 2009 iGEM competition, British_Columbia characterized BBa_I13453 in the context of a pBAD promoter family. For the results of this characterization, see here.

For the 2010 iGEM competition, Tec-Monterrey characterized BBa_I13453 again, using a construct of GFP reporter after the pBad promoter. The transfer function was modeled with a Hill equation.

Figure 1. Transfer function of BBa_I13453. Points represent individual measurements. The line is of a Hill equation fitted to our data.

(d[GFP]/dt)/OD600 = C+A*Xn/(Xn+Kn)

Experiment Characteristic Value
Transfer Function Basal rate (C) 1 d[GFP]/dt
Gain (A) 36 d[GFP]/dt
Hill coefficient (n) 2.16
Switch Point (K) 2.8 [ara] (µM)