Difference between revisions of "Part:BBa K427000"
m |
m |
||
| Line 3: | Line 3: | ||
PBad Weak Rev is the reverse complement of the Arabinose inducible promoter created by British Columbia in 2009. It is a mutation of the wild type PBad promoter that increases the amount of arabinose needed to induce the transcription of the downstream sequences. | PBad Weak Rev is the reverse complement of the Arabinose inducible promoter created by British Columbia in 2009. It is a mutation of the wild type PBad promoter that increases the amount of arabinose needed to induce the transcription of the downstream sequences. | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
It can be used to promote the transcription of DNA sequences coded in the antisense strand of DNA it can also be used to build a jammer device in which the translation of a protein is stopped because of the mRNA produced by the sense and antisense strand are complimentary. | It can be used to promote the transcription of DNA sequences coded in the antisense strand of DNA it can also be used to build a jammer device in which the translation of a protein is stopped because of the mRNA produced by the sense and antisense strand are complimentary. | ||
| + | |||
| + | This part has no special safety considerations. | ||
<!-- --> | <!-- --> | ||
Latest revision as of 10:51, 27 October 2010
PBad Weak Reverse
PBad Weak Rev is the reverse complement of the Arabinose inducible promoter created by British Columbia in 2009. It is a mutation of the wild type PBad promoter that increases the amount of arabinose needed to induce the transcription of the downstream sequences.
Usage and Biology
It can be used to promote the transcription of DNA sequences coded in the antisense strand of DNA it can also be used to build a jammer device in which the translation of a protein is stopped because of the mRNA produced by the sense and antisense strand are complimentary.
This part has no special safety considerations.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 61
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
BBa_K206001 part created by British Columbia team in 2009.